The effect of hydrodynamic shear on 3D engineered chondrocyte systems subject to direct perfusion

摘要: Bioreactors allowing direct-perfusion of culture medium through tissue-engineered constructs may overcome diffusion limitations associated with static culturing, and provide flow-mediated mechanical stimuli. The hydrodynamic stress imposed on cells within scaffolds is directly dependent scaffold microstructure bioreactor configuration. Aim this study to investigate optimal shear ranges quantitatively predict the levels during experiments. Bovine articular chondrocytes were seeded polyestherurethane foams cultured for 2 weeks in a direct perfusion designed impose 4 different values level at single flow rate (0.5 ml/min). Computational fluid dynamics (CFD) simulations carried out reconstructions obtained from micro-computed tomography images. Biochemistry analyses DNA sGAG performed, along electron microscopy. induced constructs, as estimated by CFD simulations, ranged 4.6 56 mPa. This 12-fold increase applied determined 1.7-fold mean content 2.9-fold sGAG. In contrast, sGAG/DNA ratio showed tendency decrease increasing levels. Our results suggest that condition favour synthesis engineered least beginning culture, lowest shear. conclusion, presented represent first attempt correlate invoked biosynthetic response 3D chondrocyte systems.