Hydrolytic Degradation of Nylon-6 by Pseudomonas aeruginosaHE858284 Isolated from Solid Waste Dumpsites in Lagos State, Nigeria
作者： HA Sanuth , AA Ogunjobi , OE Fagade
DOI:
关键词: Microbial biodegradation 、 Biodegradation 、 Degradation (geology) 、 Biology 、 Pseudomonas 、 Caprolactam 、 Nylon 6 、 Food science 、 Municipal solid waste 、 High-performance liquid chromatography 、 Biotechnology
摘要: Polyamide-6 (also known as nylon-6) is one of the biodegradable-resistant synthetic polymers used in manufacturing commodity plastic materials. The environmental effects persistence this material landfill pose a global problem disposal system. Knowledge microbial pattern interaction with will provide biological resources and scientific basis for development sustainable treatment method. Pseudomonas aeruginosa (HE858284), bacterial isolates from Solid Waste dumpsites that were screened their ability to utilize ?-caprolactam (the monomer sole source carbon nitrogen growth identified base on 16SrDNA gene sequences was tested nylon-6 degradation. Intermediate products culture medium monitored using High Performance Liquid Chromatography (HPLC) while biodegradation Fourier Transmittance Infrared Spectroscopy (FTIR), average number molecular mass (M n ) weight loss. An un-inoculated experiment served control. Data obtained analysed ANOVA. HPLC analysis supernatant revealed presence 6-aminohexanoic acid, some un-identified oligomers degradation fibre. changes observed FTIR spectra different functional group confirmed effect potential 12.82 % reduction M 5.23% loss within 90 days recorded (HE858284).These found be significant at p = 0.05. This bacteria isolate solid waste serves baseline information bio-treatment polymer. Key words : Nylon-6, ?-Caprolactam , dumpsite, Microbial degradation, 16SrRNA.
iiste.org参考文章(12)
D. S. Wales, B. F. Sagar, Mechanistic Aspects of Polyurethane Biodeterioration Springer, Dordrecht. pp. 351- 358 ,(1988) , 10.1007/978-94-009-1363-9_46
N.N. Baxi, A.K. Shah, ε-Caprolactam-degradation by Alcaligenes faecalis for bioremediation of wastewater of a nylon-6 production plant Biotechnology Letters. ,vol. 24, pp. 1177- 1180 ,(2002) , 10.1023/A:1016187103682
N.N. Baxi, A.K. Shah, Biological treatment of the components of solid oligomeric waste from a nylon-6 production plant World Journal of Microbiology & Biotechnology. ,vol. 16, pp. 835- 840 ,(2000) , 10.1023/A:1008971216941
Tetsuya Deguchi, Yoshihisa Kitaoka, Masaaki Kakezawa, Tomoaki Nishida, Purification and Characterization of a Nylon-Degrading Enzyme Applied and Environmental Microbiology. ,vol. 64, pp. 1366- 1371 ,(1998) , 10.1128/AEM.64.4.1366-1371.1998
Hassan Adeyemi Sanuth, Amit Yadav, Obasola Ezekiel Fagade, Yogesh Shouche, ε-Caprolactam Utilization by Proteus sp. and Bordetella sp. Isolated From Solid Waste Dumpsites in Lagos State, Nigeria, First Report. Indian Journal of Microbiology. ,vol. 53, pp. 221- 226 ,(2013) , 10.1007/S12088-013-0356-5
Jožefa Friedrich, Polona Zalar, Martina Mohorčič, Urša Klun, Andrej Kržan, Ability of fungi to degrade synthetic polymer nylon-6 Chemosphere. ,vol. 67, pp. 2089- 2095 ,(2007) , 10.1016/J.CHEMOSPHERE.2006.09.038
Anna C. Palmisano, Charles A. Pettigrew, Biodegradability of Plastics BioScience. ,vol. 42, pp. 680- 685 ,(1992) , 10.2307/1312174
M.T. Moreira, C. Palma, G. Feijoo, J.M. Lema, Decolorization of ion-exchange effluents derived from sugar-mill operations by Bjerkandera sp. BOS55 International Biodeterioration & Biodegradation. ,vol. 40, pp. 125- 129 ,(1997) , 10.1016/S0964-8305(97)00044-9
M. Sudhakar, C. Priyadarshini, Mukesh Doble, P. Sriyutha Murthy, R. Venkatesan, Marine bacteria mediated degradation of nylon 66 and 6 International Biodeterioration & Biodegradation. ,vol. 60, pp. 144- 151 ,(2007) , 10.1016/J.IBIOD.2007.02.002
Nathalie Lucas, Christophe Bienaime, Christian Belloy, Michèle Queneudec, Françoise Silvestre, José-Edmundo Nava-Saucedo, Polymer biodegradation: mechanisms and estimation techniques. Chemosphere. ,vol. 73, pp. 429- 442 ,(2008) , 10.1016/J.CHEMOSPHERE.2008.06.064