作者: R. Scott , R. Hodge , W. Paul , J. Draper
DOI: 10.1016/0168-9452(91)90281-C
关键词: Molecular cloning 、 CDNA Library Construction 、 Computational biology 、 Microspore 、 Plant science 、 Gene 、 Stamen 、 Genetics 、 Complementary DNA 、 Biology 、 Screening method
摘要: Abstract This review attempts to summarise recent progress in our understanding of androgenesis plants. There are two major divisions within this work. The first is a cytomorphological description anther development and gametogenesis model hermaphrodite intended as background against which view the molecular biological information; aspect constitutes second main division chapter. approach taken describing these advances reflect, more or less, how were made practice. Gene cloning via cDNA library construction from whole organs microspores represents technique, therefore merits approach, applied developing anther, discussed. In particular, influence screening methods on type class genes identified date highlighted. information generated gene divided roughly into three sections. describes synthesis specific mRNAs has provided valuable insights physiology ontogeny development. physical functional properties cloned cDNAs cognate expressed subject section. third sections deals with anther-expressed genes, including analysis transgenic Finally, commercial spin-off part plant science, artificial male sterility,