Identification and characterization of mouse otic sensory lineage genes.

摘要: Vertebrate embryogenesis gives rise to all cell types of an organism through the development many unique lineages derived from three primordial germ layers. The otic sensory lineage arises vesicle, a structure formed invagination placodal non-neural ectoderm. This developmental possesses differentiation potential, giving populations including hair cells, supporting and ganglion neurons auditory vestibular organs. Here we present systematic approach identify transcriptional features that distinguish (from early progenitors populations) other major vertebrate development. We used microarray analyze microdissected vesicles (embryonic day 10.5) as well isolated neonatal cochlear cells at postnatal 3. Non-otic tissue samples periotic tissues whole embryos with regions removed were reference evaluate specificity. Otic shared transcriptome-wide correlations in expression profiles members this non-otic populations. further analyzed data using comparative dimension reduction methods individual genes are specifically expressed lineage. analysis identified ranked top lineage-specific transcripts Fbxo2, Col9a2, Oc90, additional novel markers. To validate these results performed on select immunohistochemistry situ hybridization. Fbxo2 showed most striking pattern specificity lineage, robust vesicle sustained prosensory cells.