Individual cell-by-cell quantitation of lymphocyte surface membrane Ig in normal and CLL lymphocyte and during ontogeny of mouse B lymphocytes by immunoperoxidase assay.

摘要: A new quantitative immunoperoxidase method is presented for determining absolute amounts of peroxidase and, consequently, surface antigen densities individual cells in B lymphocytes from normal individuals, subjects with CLL and prolymphocytic leukemia, during ontogeny lympocytes the mouse. The following results were observed: (1) density antigenic sites lower on than lymphocytes. (2) antigens leukemic varied less cell to cell, forming a homogeneous peak histograms. (3) In very rare case CLL, was measured at time initial diagnosis (22,500 or 647 U) development blastic crisis (135,000 2576 U). by distribution changed low number per heterogeneous high cell. (4) greater much more (5) During mouse, maturation associated appearance population intermediate Smig density. finding decrease in, altered of, markers compared these ontologic findings concept that monoclonal lymphocyte may be arrested particular stage its differentiation discussed.