Variability in DNA damage of chub (Squalius cephalus L.) blood, gill and liver cells during the annual cycle

作者: K. Sunjog , S. Kolarević , M. Kračun-Kolarević , Z. Gačić , S. Skorić

DOI: 10.1016/J.ETAP.2014.03.010

关键词: Veterinary medicineBiologyGenotoxicityDNA damageAnnual cycleComet assayGillSqualiusEcologyEuropean chubAlkaline Comet Assay

摘要: Abstract In this work the genotoxic potential of water in three localities Serbia, which differ by nature and degree pollution, was determined tissues European chub ( Squalius cephalus L.) on monthly basis over 2011/2012 year season using alkaline comet assay. Specimen samples were taken from Special Nature Reserve “Uvac”, as control site, Pestan Beljanica Rivers, polluted sites at Kolubara basin, surrounded with coal mines. Three tissues, blood, gills liver used for assessing level DNA damage. Analysis done software (Comet Assay IV). The site “Uvac” showed lowest damage values all compared to Beljanica. Blood has comparison gills. Decreased observed summer, while during spring autumn increased.

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