A comparative profile of the microRNA transcriptome in immature and mature porcine testes using Solexa deep sequencing.

摘要: MicroRNAs (miRNAs) are small noncoding regulatory RNAs that play key roles in many diverse biological processes such as spermatogenesis. However, no study has been performed on the miRNA transcriptome of developing porcine testes. Here, we employed Solexa deep sequencing technology to extend repertoire testis miRNAs and extensively compare expression patterns sexually immature mature two RNA libraries derived from (30 days) (180 pig samples yielded over 25 million high-quality reads. Overall, developmental stages had significantly different compositions. A custom data analysis pipeline identified 398 known and/or homologous conserved miRNAs, 15 novel pig-specific 56 candidate miRNAs. We further observed multiple variants a new bidirectional transcribed locus, ssc-mir-181a. total 122 were differentially expressed testes, 10 validated using quantitative RT-PCR. Furthermore, GO KEGG pathway analyses predicted targets illustrate likely for these This is first comparative profile testes approach, it provides useful resource future studies role spermatogenesis male infertility treatment.