Distribution and pharmacology of alanine–serine–cysteine transporter 1 (asc‐1) in rodent brain

摘要: A polyclonal antibody against the Na + -independent alanine-serine-cysteine transporter 1 (asc-1) was raised and specificity of verified by Western blots performed on membranes prepared from HEK293 cells transiently transfected with cloned murine asc-1. The then used to localize in brain two rodent species using immunohistochemistry at light electron microscopical level. asc-1-immunoreactivity (asc-1-ir) widely distributed throughout mouse rat brain. Areas high levels asc-1-ir included hypothalamus, medial septal area, globus pallidus, entopeduncular nucleus, cingulate retrosplenial cortices. Moderate observed several areas including layers III V neocortex, thalamus, nucleus accumbens, caudate putamen, bed stria terminalis, all amygdaloid nuclei, hippocampus (CA1-CA3 hilus dentate gyrus), as well brainstem nuclei. asc-1 -ir punctuate staining consistent varicosities matching neuronal cell bodies dendritic fields. At ultrastructural level, mainly confined presynaptic terminals. Immunostaining either glial or perivascular sites not white matter completely devoid asc-1-ir. Furthermore, pharmacology uptake site for [ 3 H]D-serine synaptosomal P2 fractions compared substrate human a degree correlation demonstrated. We conclude that is widespread limited structures may contribute synaptic clearance D-serine