Histamine H4 receptor stimulation suppresses IL-12p70 production and mediates chemotaxis in human monocyte-derived dendritic cells.

摘要: There is increasing evidence that histamine as an important mediator of immediate type allergic reactions also effects professional APCs. Recent reports showed on human monocyte-derived dendritic cells (MoDC) mediated primarily via H1 receptors (H1R) and H2R. We show here MoDC express H3R H4R at the mRNA protein level. down-regulated up-regulated during differentiation from monocytes to MoDC. or H2R stimulation suppressed IL-12p70 production in Induction cAMP was necessary for inhibition In contrast, did not affect but induced transcription factor AP-1, U0126, inhibitor AP-1 transactivation MEK, rescued suppression. Moreover, responded a agonist (and agonist) with increased F-actin polymerization migration modified Boyden chamber assays, suggesting chemotactic effect H4R. Thus, results immunomodulatory effects. Histamine induces chemotaxis suppression different using signaling pathways, which might be pathogenesis therapeutic interventions diseases.