Down-regulation of thyroxine-binding globulin messenger ribonucleic acid by 3,5,3'-triiodothyronine in human hepatoblastoma cells.
作者： T C Crowe , N L Cowen , N M Loidl , D J Topliss , J R Stockigt
DOI: 10.1210/JCEM.80.7.7608285
关键词: Endocrinology 、 Triiodothyronine 、 Secretion 、 Thyroxine-binding globulin 、 Hormone 、 Binding protein 、 Internal medicine 、 Messenger RNA 、 Biology 、 Globulin 、 Cell culture
摘要: A sensitive [125I]-T4 binding assay was used to measure serum T4-binding globulin (TBG) in 60 individuals selected on the basis of their total circulating T3 concentrations, and a relationship between TBG thyroid hormone levels humans confirmed. There significant correlation or free T4 index. secretion messenger ribonucleic acid (mRNA) production were studied with continuous culture human hepatoblastoma cell line, HepG2. Cells maintained serum-free media for experimental manipulations. The addition 100 nmol/L medium resulted time-dependent down-regulation mRNA 33 +/- 6% (+/- SD, n = 4) untreated control by 24 h. Suppression first detectable at 8 h (57% levels). effect dose-responsive, half-maximal suppression occurring bioavailable concentration approximately 30 pmol/L. not caused change stability. Proteins secreted HepG2 cells bound an affinity identical that normal TBG. Cell parallel protein consistent rate 50 ng/10(6) per day. Variations presence corresponded changes observed mRNA. These data show is negatively correlated vivo. corresponding suggests this result action cellular synthesis.
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