Fluorescence correlation methods for imaging cellular behavior of sphingolipid-interacting probes.

摘要: For cell biologists interested in the properties of membranes, their composition, and dynamics, realization that sphingolipids cholesterol have capacity to self-organize into ordered domains has given rise a need visualize these lipids actual living membranes. In order find out how various classes distribute membrane what behaviors are, it is extremely useful apply fluorescent probes either interact with lipids, or themselves behave like naturally occurring lipids. At same time, imaging modalities observe require appropriate spatial temporal resolution, on milli- microsecond timescale. Knowledge organization changes during processes signaling invasion by pathogens will undoubtedly be relevant our understanding action infectious diseases, bacterial toxins, even disease pathologies prion Alzheimer's disease, where glycosphingolipids (GSL) sphingolipid-rich act as receptors docking sites. such cases, composition dynamics clearly play role infectivity. The present challenge coming up high-resolution non-invasive approaches observing dynamic structural features sphingolipid-containing domains. This chapter discuss several variants applications fluorescence correlation spectroscopy well can used study sphingolipid dynamics.