Three-dimensional architecture of collagen type VI in the human trabecular meshwork.

摘要: PURPOSE Type VI collagen is a primary component of the extracellular matrix many connective tissues. It can form distinct aggregates depending on tissue structure, chemical environment, and physiology. In current study we examine ultrastructure mode aggregation type molecules in human trabecular meshwork. METHODS Trabecular meshwork was dissected from donor eyes, three-dimensional transmission electron microscopy performed. RESULTS Electron-dense structures were detected identified as assemblies based spatial arrangement molecules, 105-nm axial periodicity themselves, their characteristic double bands, which arose globular domains molecules. Sulfated proteoglycans also seen to associate with either domain or inner rod-like segments tetramers. CONCLUSIONS No extended structural regularity organization within evident, lateral separation tetramers forming varied, did angle formed by main axes adjacent This potentially reflective specific nature facilitates aqueous outflow eye, speculate that ions proteins might prevent more tight packing assemblies.