Experimental formation of 100 nm periodic fibrils in the mouse corneal stroma and trabecular meshwork.

摘要: The so-called long-spacing collagen that exhibits approximately 100 nm periodicity has been observed in the interstitial connective tissue of various organs under normal and pathological conditions. Although we, as well many other investigators, have reported an increasing amount these structures with age human trabecular meshwork, significance mechanism its formation are still unknown. We incubated mouse ocular tissues culture medium containing 20 mM ATP prepared them for electron microscopic observation according to method Bruns et al (J Cell Biol 103:393, 1986). After incubation, abundant periodic fibrils were corneal stroma region both which show no structure For experimental fibrils, ATP, acidic condition temperature around 37 degrees C necessary. our experiment very similar collagen, dark transverse bands intervals fine filaments 6-7 diameter axially connect bands. Long-spacing is not usually cornea, even aged persons. results study suggest occurrence related special conditions developing meshwork but stroma. Experimental studies fibril mice offer a useful model age-related increase eye.