Tracking genetic invasions: Genome‐wide single nucleotide polymorphisms reveal the source of pyrethroid‐resistant Aedes aegypti (yellow fever mosquito) incursions at international ports

摘要: Biological invasions are increasing globally in number and extent despite efforts to restrict their spread. Knowledge of incursion pathways is necessary prevent new design effective biosecurity protocols at source recipient locations. This study uses genome-wide single nucleotide polymorphisms (SNPs) determine the origin 115 incursive Aedes aegypti(yellow fever mosquito) detected international ports Australia New Zealand. We also genotyped mosquitoes three point mutations voltage-sensitive sodium channel (Vssc) gene: V1016G, F1534C S989P. These confer knockdown resistance synthetic pyrethroid insecticides, widely used for controlling invertebrate pests. first delineated reference populations using Ae. aegypti sampled from 15 locations Asia, South America, Pacific Islands. Incursives were assigned these discriminant analysis principal components (DAPC) an assignment test with a support vector machine predictive model. Bali, Indonesia, was most common Australia, while Zealand originated Islands such as Fiji. Most incursives had same allelic genotype across Vsscgene mutations, which confers strong pyrethroids, only insecticide class current, implemented aircraft disinsection endorsed by World Health Organization (WHO). Additionally, all internationally Vssc linked that not found Australian populations. findings demonstrate preventing introductions invertebrates must consider resistance, highlight usefulness genomic data sets managing global objectives.