A CDC2‐Related Kinase is Associated with Macronuclear DNA Synthesis in Paramecium tetraurelia
作者： LIREN TANG , SINA M. ADL , JAMES D. BERGER
DOI: 10.1111/J.1550-7408.1997.TB05711.X
关键词: Cell division 、 Macronucleus 、 Cyclin-dependent kinase 、 Paramecium tetraurelia 、 Kinase activity 、 Biochemistry 、 Cyclin-dependent kinase 1 、 Kinase 、 MAP2K7 、 Biology
摘要: Cell division in higher eukaryotes is mainly controlled by CDK (serine/threonine) protein kinases and other components of these kinase complexes. Previously, we showed homologous also occur the ciliate Paramecium tetraurelia. In this report, a polyclonal antibody was produced against GST fusion which contained N-terminal third previously isolated CDC2PTA protein. It recognized 36 kDa polypeptide on western blots did not cross-react with related 34 polypeptide. This no affinity for yeast P13 suc1 The level invariant throughout cell cycle decreased slightly when cells entered stationary phase. Indirect immunofluorescence localized macronucleus, but micronuclei. Native immunoprecipitated activity assayed using histone H1 as substrate elutriation synchronized samples. high during early stages reached peak at 2.5 h after elutriation, corresponds to time initiation macronuclear DNA synthesis. suggests may be associated regulation These results allow us draw concrete comparisons first between role ciliates eukaryotes.
sci-hub.se 参考文章(48)
Matthew Meyerson, Greg H Enders, Chin-Lee Wu, LK Su, C Gorka, C Nelson, Ed Harlow, LH Tsai, A family of human cdc2-related protein kinases. The EMBO Journal. ,vol. 11, pp. 2909- 2917 ,(1992) , 10.1002/J.1460-2075.1992.TB05360.X
L. Dirick, T. Böhm, K. Nasmyth, Roles and regulation of Cln-Cdc28 kinases at the start of the cell cycle of Saccharomyces cerevisiae. The EMBO Journal. ,vol. 14, pp. 4803- 4813 ,(1995) , 10.1002/J.1460-2075.1995.TB00162.X
Parry D, Dickson C, Peters G, Holder A, Bates S, MacAllan D, Bonetta L, CDK6 (PLSTIRE) and CDK4 (PSK-J3) are a distinct subset of the cyclin-dependent kinases that associate with cyclin D1. Oncogene. ,vol. 9, pp. 71- 79 ,(1994)
Jacqueline Hayles, Stephen Aves, Paul Nurse, suc1 is an essential gene involved in both the cell cycle and growth in fission yeast. The EMBO Journal. ,vol. 5, pp. 3373- 3379 ,(1986) , 10.1002/J.1460-2075.1986.TB04653.X
Y WATANABE, M IKEDA, Further confirmation of "division protein" fraction in Tetrahymena pyriformis. Experimental Cell Research. ,vol. 39, pp. 464- 469 ,(1965) , 10.1016/0014-4827(65)90049-2
Graña X, Reddy Ep, Cell cycle control in mammalian cells : role of cyclins, cyclin dependent kinases (CDKs), growth suppressor genes and cyclin-dependent kinase inhibitors (CKIs) Oncogene. ,vol. 11, pp. 211- 219 ,(1995)
James D. Berger, The Cell Cycle and Regulation of Cell Mass and Macronuclear DNA Content Paramecium. pp. 97- 119 ,(1988) , 10.1007/978-3-642-73086-3_7
Ada S.-L. Ching, James D. Berger, Control of cell division in Paramecium tetraurelia. Effects of abrupt changes in nutrient level on accumulation of macronuclear DNA and cell mass. Experimental Cell Research. ,vol. 167, pp. 191- 202 ,(1986) , 10.1016/0014-4827(86)90216-8
J Hindley, G Phear, M Stein, D Beach, Sucl+ encodes a predicted 13-kilodalton protein that is essential for cell viability and is directly involved in the division cycle of Schizosaccharomyces pombe. Molecular and Cellular Biology. ,vol. 7, pp. 504- 511 ,(1987) , 10.1128/MCB.7.1.504
H Matsushime, D E Quelle, S A Shurtleff, M Shibuya, C J Sherr, J Y Kato, D-type cyclin-dependent kinase activity in mammalian cells. Molecular and Cellular Biology. ,vol. 14, pp. 2066- 2076 ,(1994) , 10.1128/MCB.14.3.2066