Gd(III) ion-chelated supramolecular assemblies composed of PGMA-based polycations for effective biomedical applications

摘要: Ethanolamine (EA) or ethylenediamine (ED)-functionalized poly(glycidyl methacrylate) (PGMA), namely PGEA PGED, has recently been used as effective gene carriers because of their low cytotoxicity and high transfection efficiency. In this study, a series PGMA-based supramolecular polycations (PGED-Gd@PGEAs) with magnetic resonance imaging (MRI) functions were readily constructed by assembling multiple adamantine-headed star (Ad-PGEA) units versatile PGED-CD-Gd backbone, which possessed numerous flanking β-cyclodextrin species Gd3+ ions. The properties different PGED-Gd@PGEA vectors systematically characterized, including the plasmid DNA condensation ability, cytotoxicity, efficiency, cellular uptake MRI function. Such had lower toxicity than ‘gold standard’ polyethylenimine (PEI, 25 kDa). Furthermore, PGED-Gd@PGEAs exhibited significantly higher efficiencies PEI constituent (PGED-CD-Gd Ad-PGEA). chelation ions imparted good ability without obvious adverse effects. present design would provide useful information for development low-toxicity high-efficiency multifunctional delivery systems. An intricate polymer complex can carry genes to targets efficiency toxicity. Gene therapy potential revolutionize treatment diseases such cancer, but non-viral systems are needed ensure patient safety. Fu-Jian Xu from Beijing University Chemical Technology co-workers have taken steps reach goal cationic polymers gadolinium (Gd) into spherical, host-guest complexes. These compounds, based on biocompatible cyclodextrin molecules, be size adjusted nanoparticles correct dimensions optimal uptake. addition Gd turns device probe in vivo monitoring nucleic acid transfer. A new methacrylate)-based designed