Specific Imaging of Inflammation with the 18kDa Translocator Protein Ligand DPA-714 in Animal Models of Epilepsy and Stroke

摘要: Inflammation is a pathophysiological hallmark of many diseases the brain. Specific imaging cells and molecules that contribute to cerebral inflammation therefore highly desirable, both for research in clinical application. The 18 kDa translocator protein (TSPO) has been established as suitable target detection activated microglia/macrophages. A number novel TSPO ligands have developed recently. Here, we evaluated high affinity ligand DPA-714 marker brain two independent animal models. For first time, specificity radiolabeled microglia/macrophages was studied rat model epilepsy (induced using Kainic acid) mouse stroke (transient middle artery occlusion, tMCAO) high-resolution autoradiography immunohistochemistry. Additionally, cold-compound blocking experiments were performed changes blood-brain barrier (BBB) permeability determined. Target-to-background ratios 2 3 achieved lesioned vs. unaffected tissue tMCAO models, respectively. In uptake into lesion corresponded well with extent Ox42- or Iba1-immunoreactive model, almost completely blocked by pre-injection FEDAA1106, another high-affinity ligand. Ligand degree BBB opening size model. We provide further strong evidence specific image experimental models inflammation.