Bio-corrosion of stainless steel by osteoclasts-in vitro evidence

摘要: Most metals in contact with biological systems undergo corrosion by an electrochemical process. This study investigated whether human osteoclasts (OC) are able to grow on stainless steel (SS) and directly corrode the metal alloy leading formation of corresponding ions, which may cause inflammatory reactions activate immune system. Scanning electron microscopy analysis demonstrated long-term viable OC cultures evident resorption features surface SS discs were cultured for 21 days. The findings confirmed atomic emission spectrometry investigations showing significantly increased levels chromium, nickel, manganese supernatant cultures. Furthermore, significant pro-inflammatory cytokines IL-1beta, IL-6, TNF-alpha, considered be major mediators osteolysis, revealed same cytometric bead array analysis. Within present study, it was shown that osteoclast precursors differentiate towards mature SS. cells release induce secretion known enhance differentiation, activation, survival. Enhanced subsequently released ions therefore result enhanced osteolytic lesions peri-prosthetic bone, contributing aseptic loosening implant.