Understanding the tissue effects of tribo‐corrosion: Uptake, distribution, and speciation of cobalt and chromium in human bone cells

摘要: Cobalt and chromium species are released in the local tissues as a result of tribo-corrosion, affect bone cell survival function. However we have little understanding mechanisms cellular entry, intracellular distribution, speciation metals that impaired health. Here used synchrotron based X-ray fluorescence (XRF), absorption spectroscopy (XAS), fluorescent-probing approaches candidate receptors P2X7R divalent metal transporter-1 (DMT-1), to better understand intra-cellular distribution cobalt (Co) (Cr) human osteoblasts primary osteoclasts. We found both Co Cr were most highly localized at nuclear perinuclear sites osteoblasts, suggesting uptake through membrane transporters, supported by finding P2X7 receptor blockade reduced entry Co. In contrast, present discrete corresponding basolateral osteoclasts, endocytosis trafficking functional secretory domain. An reduction Cr6+ Cr3+ was only redox change observed cells treated with Co2+, Cr3+, Cr6+. Our data suggest processing differs between © 2014 The Authors. Journal Orthopaedic Research published Wiley Periodicals, Inc. on behalf Society. J Orthop Res 33:114–121, 2015.