Methods of staining target chromosomal DNA employing high complexity nucleic acid probes
作者： Ol'li-Pekka Kallioniemi , Daniel Pinkel , Joe W. Gray , Anne Kallioniemi , Masaru Sakamoto
DOI:
关键词: Stain 、 Staining 、 Cytogenetics 、 Retinoblastoma 、 Metaphase 、 Biology 、 Chronic myelogenous leukemia 、 Nucleic acid sequence 、 In situ hybridization 、 Molecular biology
摘要: Methods and compositions for staining based upon nucleic acid sequence that employ probes are provided. Said methods produce patterns can be tailored specific cytogenetic analyses. appropriate in situ hybridization stain both interphase metaphase chromosomal material with reliable signals. The typically of a complexity greater than 50 kb, the depending application. reagents provided detection genetic rearrangements. Probes test kits use detecting rearrangements, particularly tumor cytogenetics, disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML), retinoblastoma, ovarian uterine cancers, biological dosimetry. described research, differentiation cytogenetically similar but genetically different diseases, many prognostic diagnostic applications.
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