Influence of the curvature on the water structure in the headgroup region of phospholipid bilayer studied by the solvent relaxation technique
作者： Jan Sýkora , Piotr Jurkiewicz , Richard M. Epand , Ruud Kraayenhof , Marek Langner
DOI: 10.1016/J.CHEMPHYSLIP.2005.03.003
关键词: Stokes shift 、 Bilayer 、 Vesicle 、 Lipid bilayer 、 Analytical chemistry 、 Curvature 、 Membrane curvature 、 Bound water 、 Relaxation (NMR) 、 Chemistry
摘要: Solvent relaxation (SR) in 1,2-dioleoyl-palmitoyl-sn-glycero-3-phosphocholine (DOPC) unilamellar vesicles of different size was probed by 6-hexadecanoyl-2-(((2-(trimethylammonium)ethyl)methyl)amino)naphthalene chloride (Patman), 6-propionyl-2-dimethylaminonaphthalene (Prodan) and 4-[(n-dodecylthio)methyl]-7-(N,N-dimethylamino)-coumarin (DTMAC). Patman probes the amount mobility bound water molecules located at carbonyl region bilayer. Membrane curvature significantly accelerates solvent process, but does not influence total Stokes shift, showing that membrane increases mobility, without affecting present headgroup region. This pattern also verified for other phosphatidylcholines. Prodan is phosphate bilayer a more polar, mobile heterogeneous environment than Patman. The on SR similar, however, less pronounced compared to DTMAC (first time used SR) shows broad distribution locations along z-axis. A substantial coumarin chromophores face bulk water. No effect detectable.
core.ac.uk
narcis.nl
elsevier.com参考文章(25)
Wieland B Huttner, Joshua Zimmerberg, Implications of lipid microdomains for membrane curvature, budding and fission. Current Opinion in Cell Biology. ,vol. 13, pp. 478- 484 ,(2001) , 10.1016/S0955-0674(00)00239-8
E.G. Finer, A. Darke, Phospholipid hydration studied by deuteron magnetic resonance spectroscopy Chemistry and Physics of Lipids. ,vol. 12, pp. 1- 16 ,(1974) , 10.1016/0009-3084(74)90064-4
R. Hutterer, F.W. Schneider, M. Hof, Anisotropy and lifetime profiles for n-anthroyloxy fatty acids: a fluorescence method for the detection of bilayer interdigitation Chemistry and Physics of Lipids. ,vol. 86, pp. 51- 64 ,(1997) , 10.1016/S0009-3084(97)02659-5
R. Hutterer, F.W. Schneider, H. Sprinz, M. Hof, Binding and relaxation behaviour of prodan and patman in phospholipid vesicles: a fluorescence and 1H NMR study Biophysical Chemistry. ,vol. 61, pp. 151- 160 ,(1996) , 10.1016/S0301-4622(96)02185-0
Jan Sýkora, Virima Mudogo, Rudi Hutterer, Miloš Nepras, Jozef Vaněrka, Petr Kapusta, Vlastimil Fidler, Martin Hof, ABA-C15: A new dye for probing solvent relaxation in phospholipid bilayers Langmuir. ,vol. 18, pp. 9276- 9282 ,(2002) , 10.1021/LA026435C
Parkson Lee Gau Chong, Sarah Capes, Patrick T. T. Wong, Effects of hydrostatic pressure on the location of PRODAN in lipid bilayers: a FT-IR study. Biochemistry. ,vol. 28, pp. 8358- 8363 ,(1989) , 10.1021/BI00447A014
Joshua Zimmerberg, Stuart McLaughlin, Membrane curvature: how BAR domains bend bilayers. Current Biology. ,vol. 14, ,(2004) , 10.1016/J.CUB.2004.02.060
Andrey S. Klymchenko, Guy Duportail, Alexander P. Demchenko, Yves Mély, Bimodal Distribution and Fluorescence Response of Environment-Sensitive Probes in Lipid Bilayers Biophysical Journal. ,vol. 86, pp. 2929- 2941 ,(2004) , 10.1016/S0006-3495(04)74344-1
M.J. Hope, M.B. Bally, L.D. Mayer, A.S. Janoff, P.R. Cullis, Generation of multilamellar and unilamellar phospholipid vesicles Chemistry and Physics of Lipids. ,vol. 40, pp. 89- 107 ,(1986) , 10.1016/0009-3084(86)90065-4
R.S. Fee, M. Maroncelli, Estimating the time-zero spectrum in time-resolved emmsion measurements of solvation dynamics Chemical Physics. ,vol. 183, pp. 235- 247 ,(1994) , 10.1016/0301-0104(94)00019-0