Bombardier Enables Delivery of Short-Form Bomanins in the Drosophila Toll Response

摘要: Toll mediates a robust and effective innate immune response across vertebrates invertebrates. In Drosophila melanogaster, activation of by systemic infection drives the accumulation rich repertoire effectors in hemolymph, including recently characterized Bomanins, as well classical antimicrobial peptides (AMPs). Here we report functional characterization Toll-induced hemolymph protein encoded bombardier (CG18067) gene. Using CRISPR/Cas9 system to generate precise deletion transcriptional unit, found that Bombardier is required for Toll-mediated defense against fungi Gram-positive bacteria. Assaying cell-free Bomanin-dependent candidacidal activity also dependent on Bombardier, but independent antifungal AMPs Drosomycin Metchnikowin. mass spectrometry, demonstrated results specific absence short-form Bomanins from hemolymph. addition, flies lacking exhibited defect pathogen tolerance trace an aberrant condition triggered activation. These lead us model which presence wild-type enables proper folding, secretion, or intermolecular associations disrupts one more these steps, resulting defects both resistance tolerance.