Antibodies against GM1 ganglioside affect K+ and Na+ currents in isolated rat myelinated nerve fibers.

摘要: High titers of anti-GM1 ganglioside antibodies (anti-GM1 antibodies) may be implicated in lower motor neuron disease. We studied the pathogenic role antibody using petroleum jelly-gap voltage clamp technique on isolated single myelinated rat nerve fibers. Anti-GM1 antisera were obtained from rabbits immunized with GM1 ganglioside. Extracellularly applied without complement activity increased both rate rise and amplitude K+ current elicited by step depolarization, little effect Na+ current. In presence active complement, however, decreased current, caused a progressive increase nonspecific leakage Neither alone nor complement-supplemented which depleted affinity chromatography had any ionic These observations indicate that themselves can uncover channels paranodal region, while bound to nodal membrane form antibody-complement complexes block disrupt at node Ranvier.