The isolation of mitochondria and mitochondrial DNA
作者： Helen T. Nivison , Phillip E. McClean , Maureen R. Hanson , Mary A. O'Connell , Maury L. Boeshore
DOI: 10.1016/0076-6879(86)18091-8
关键词: Biochemistry 、 DNA 、 Nucleic acid 、 Mitochondrial DNA 、 Organelle 、 Plastid 、 Molecular biology 、 Ribosome 、 Protein biosynthesis 、 Biology 、 Mitochondrion
摘要: Publisher Summary This chapter describes the isolation procedures for obtaining mitochondrial DNA (mtDNA) from three tissue types of several genera dicot plants. It discusses suspension culture mitochondria capable active in organello protein synthesis. Alternative procedures, including analysis mtDNAs present total preparations, dye-mediated gradient separations organelle DNAs, and use other sources are also discussed. The key criterion a successful preparation is freedom contaminating plastid nuclear DNAs cytoplasmic ribosomes. Significant contamination with subcellular particles can be tolerated provided these do not contain nucleic acids or ribosomes A feature differential centrifugation to separate bulk nuclei, plastids, cellular debris fraction that differs particle size density.
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