A comparative proteomic analysis of Bacillus coagulans in response to lactate stress during the production of l-lactic acid

作者: Xiuwen Wang , Jiayang Qin , Landong Wang , Ping Xu

DOI: 10.1007/S10529-014-1639-6

关键词: Lactate dehydrogenaseBacillus coagulansReductaseBiologyCysteine synthaseBiochemistrySodium lactateLactic acidCalciumLactic acid fermentation

摘要: The growth rate and maximum biomass of Bacillus coagulans 2–6 were inhibited by lactate; inhibition sodium lactate was stronger than calcium lactate. differences protein expressions B. under the stress determined using two-dimensional electrophoresis coupled with mass spectrometric identification. Under non-stress condition, stress, number detected spots 1,571 ± 117, 1,281 231 904 127, respectively. Four proteins high expression identified: dehydrogenase, cysteine synthase A, aldo/keto reductase ribosomal L7/L12. These are thus potential targets for reconstruction to promote its resistance stress.

参考文章(15)
Dong-Mei Bai, Qiang Wei, Zhi-Hui Yan, Xue-Ming Zhao, Xin-Gang Li, Shi-Min Xu, Fed-batch fermentation of Lactobacillus lactis for hyper-production of L-lactic acid. Biotechnology Letters. ,vol. 25, pp. 1833- 1835 ,(2003) , 10.1023/A:1026276925649
Jiayang Qin, Xiuwen Wang, Zhaojuan Zheng, Cuiqing Ma, Hongzhi Tang, Ping Xu, Production of L-lactic acid by a thermophilic Bacillus mutant using sodium hydroxide as neutralizing agent. Bioresource Technology. ,vol. 101, pp. 7570- 7576 ,(2010) , 10.1016/J.BIORTECH.2010.04.037
Yin Li, Jeroen Hugenholtz, Tjakko Abee, Douwe Molenaar, Glutathione Protects Lactococcus lactis against Oxidative Stress Applied and Environmental Microbiology. ,vol. 69, pp. 5739- 5745 ,(2003) , 10.1128/AEM.69.10.5739-5745.2003
Kenji Okano, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo, Biotechnological production of enantiomeric pure lactic acid from renewable resources: recent achievements, perspectives, and limits. Applied Microbiology and Biotechnology. ,vol. 85, pp. 413- 423 ,(2010) , 10.1007/S00253-009-2280-5
Avelino Corma, Sara Iborra, Alexandra Velty, Chemical Routes for the Transformation of Biomass into Chemicals Chemical Reviews. ,vol. 107, pp. 2411- 2502 ,(2007) , 10.1021/CR050989D
Jiayang Qin, Bo Zhao, Xiuwen Wang, Limin Wang, Bo Yu, Yanhe Ma, Cuiqing Ma, Hongzhi Tang, Jibin Sun, Ping Xu, Non-Sterilized Fermentative Production of Polymer-Grade L-Lactic Acid by a Newly Isolated Thermophilic Strain Bacillus sp. 2–6 PLoS ONE. ,vol. 4, pp. e4359- ,(2009) , 10.1371/JOURNAL.PONE.0004359
Rathin Datta, Michael Henry, Lactic acid: recent advances in products, processes and technologies - a review Journal of Chemical Technology & Biotechnology. ,vol. 81, pp. 1119- 1129 ,(2006) , 10.1002/JCTB.1486
Rojan P. John, K. Madhavan Nampoothiri, Ashok Pandey, Fermentative production of lactic acid from biomass: an overview on process developments and future perspectives Applied Microbiology and Biotechnology. ,vol. 74, pp. 524- 534 ,(2007) , 10.1007/S00253-006-0779-6
Naresh K. Budhavaram, Zhiliang Fan, Production of lactic acid from paper sludge using acid-tolerant, thermophilic Bacillus coagulan strains. Bioresource Technology. ,vol. 100, pp. 5966- 5972 ,(2009) , 10.1016/J.BIORTECH.2009.01.080