Transgressive segregation reveals two Arabidopsis TIR-NB-LRR resistance genes effective against Leptosphaeria maculans, causal agent of blackleg disease.
作者： Jens Staal , Maria Kaliff , Svante Bohman , Christina Dixelius
DOI: 10.1111/J.1365-313X.2006.02688.X
关键词: Gene 、 Leptosphaeria maculans 、 Quantitative trait locus 、 R gene 、 Chromosome 4 、 Biology 、 Transgressive segregation 、 Mutant 、 Sequence analysis 、 Genetics
摘要: In a cross between the two resistant accessions Col-0 and Ler-0, 15:1 segregation was found in F2, suggesting presence of unlinked resistance loci to Leptosphaeria maculans. One hundred Col-4 x 50 Ler-2 Cvi-1 recombinant inbred lines, seven susceptible Ler-0 Ws-0 F2 progenies were examined identify loci. Resistance Col-4, (RLM1) mapped marker m305 on chromosome 1. mapping populations located RLM2(Ler) same arm 4. A tight physical location RLM2 established through near-isogenic lines. This region correspond an ancient duplication event RLM1 Two independent T-DNA mutants TIR-NB-LRR R gene (At1g64070) displayed susceptibility, L. maculans mutant phenotypes confirmed be allelic for rlm1 F1 after crosses with rlm1(Ler)rlm2(Col) plants. Complementation genomic sequence At1g64070 conferred resistance. addition, neighbouring homologous (At1g63880) moderate susceptibility Sequence analysis revealed that truncated by premature stop codon, At1g63880 absent Ler-0. RNA interference is dependent genes structurally related RLM1. Camalexin identified as quantitative co-dominant factor origin, but RLM1/RLM2 was, however, require RAR1 partially HSP90.1.
sci-hub.se 参考文章(66)
Yule Liu, Michael Schiff, Giovanna Serino, Xing-Wang Deng, S. P. Dinesh-Kumar, Role of SCF ubiquitin-ligase and the COP9 signalosome in the N gene-mediated resistance response to Tobacco mosaic virus. The Plant Cell. ,vol. 14, pp. 1483- 1496 ,(2002) , 10.1105/TPC.002493
Blake C. Meyers, Michele Morgante, Richard W. Michelmore, TIR-X and TIR-NBS proteins: two new families related to disease resistance TIR-NBS-LRR proteins encoded in Arabidopsis and other plant genomes. Plant Journal. ,vol. 32, pp. 77- 92 ,(2002) , 10.1046/J.1365-313X.2002.01404.X
P. A. Delwiche, P. H. Williams, Screening for resistance to blackleg of crucifers in the seedling stage Cruciferae Newsletter. ,vol. 4, pp. 24- ,(1979)
ZB Zeng, CJ Basten, S Wang, Windows QTL Cartographer 2·5 ,(2011)
I. A. Kagan, R. Hammerschmidt, Arabidopsis ecotype variability in camalexin production and reaction to infection by Alternaria brassicicola. Journal of Chemical Ecology. ,vol. 28, pp. 2121- 2140 ,(2002) , 10.1023/A:1021020512846
Naohiro Inohara, Gabriel Nuñez, NODs: intracellular proteins involved in inflammation and apoptosis. Nature Reviews Immunology. ,vol. 3, pp. 371- 382 ,(2003) , 10.1038/NRI1086
Figen Mert-Türk, Mark H Bennett, John W Mansfield, Eric B Holub, Camalexin accumulation in Arabidopsis thaliana following abiotic elicitation or inoculation with virulent or avirulent Hyaloperonospora parasitica Physiological and Molecular Plant Pathology. ,vol. 62, pp. 137- 145 ,(2003) , 10.1016/S0885-5765(03)00047-X
Paola Veronese, Xi Chen, Burton Bluhm, John Salmeron, Robert Dietrich, Tesfaye Mengiste, The BOS loci of Arabidopsis are required for resistance to Botrytis cinerea infection Plant Journal. ,vol. 40, pp. 558- 574 ,(2004) , 10.1111/J.1365-313X.2004.02232.X
Clare Lister, Caroline Dean, Recombinant inbred lines for mapping RFLP and phenotypic markers in Arabidopsis thaliana Plant Journal. ,vol. 4, pp. 745- 750 ,(1993) , 10.1046/J.1365-313X.1993.04040745.X
Barbara J. Howlett, Current knowledge of the interaction between Brassica napus and Leptosphaeria maculans Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie. ,vol. 26, pp. 245- 252 ,(2004) , 10.1080/07060660409507141