Camalexin accumulation in Arabidopsis thaliana following abiotic elicitation or inoculation with virulent or avirulent Hyaloperonospora parasitica

摘要: Abstract Wild type accessions of Arabidopsis (Col-0, Nd-1 and Ws-3) were compared with phytoalexin-deficient mutants (Col- pad1 , - pad2 pad3 pad4 ) the enhanced disease susceptibility mutant Ws- eds1 for camalexin accumulation following inoculation virulent or avirulent isolates Hyaloperonospora parasitica (downy mildew), treatment abiotic elicitor AgNO 3 . Camalexin was greatest in Ws-3 all induction treatments to other wild accessions, indicating that natural genetic variation exists regulation biosynthesis. In Col-0, more accumulated plants inoculated H. isolate (Maks9) than (Hiks1, recognized by RPP7 ). at least two-fold less Col-0 isolate; a similar reduction (but difference) observed No detected biosynthesis pathogen inoculation; but traces identified treatment. Equivalent levels as treatments. Results from confirmed EDS1 protein is required regulating both RPP1 -mediated downy mildew resistance production. The combined results suggest most likely consequence caused does not therefore confer primary infection this parasite. It also appears signalling case involves PAD2, PAD4 proteins necessarily PAD1.