Development of a DNA macroarray for simultaneous detection of multiple foodborne pathogenic bacteria in fresh chicken meat.

摘要: A DNA macroarray was developed to provide the ability detect multiple foodborne pathogens in fresh chicken meat. Probes targeted 16S rRNA and genus- species-specific genes, including fimY, ipaH, prfA, uspA, were selected for specific detection of Salmonella spp., Shigella Listeria monocytogenes, Escherichia coli, respectively. The combination target gene amplification by PCR a our system able distinguish all bacteria from pure cultures with sensitivity 105 c.f.u. ml−1. also applied 10 meat samples. assay validation demonstrated that combining enrichment steps macroarray, 4 could be detected simultaneously L. monocytogenes boydii samples at least 3 initial contamination 25 g samples, advantages protocol are high accuracy time reduction when compared conventional culture. investigation cost effective modern oligonucleotide microarray techniques because there no expensive equipment required pathogens.