Surface functionalization of microgrooved titanium with dual growth factor-releasing nanoparticles for synergistic osteogenic differentiation of human mesenchymal stem cells

作者： Suk Won Lee , Hong Jae Lee , Jae Won Lee , Kyung-Hee Kim , Jong-Ho Kang

DOI: 10.1016/J.COLSURFB.2015.08.011

关键词: Chemistry 、 Contact angle 、 Bone morphogenetic protein 2 、 Surface modification 、 Nanoparticle 、 Biomaterial 、 Osteoblast 、 Nanotechnology 、 Titanium 、 Fluorescence microscope 、 Biophysics

摘要: We demonstrate that dual release of bone morphogenic protein-2 (BMP-2) and insulin-like growth factor-1 (IGF-1) by catechol-functionalized adhesive polymer nanoparticles on microgrooved titanium (Ti) surface enhances in vitro osteoblastic differentiation human mesenchymal stem cells (MSCs). The consisted three distinct domains, Ti-adhesive catechol groups, anionic poly(L-aspartic acid) (PAsp) shells, hydrophobic poly(L-phenylalanine) (PPhe) cores. immobilization the onto Ti was verified using various analytical tools, such as field-emission scanning electron microscopy (Fe-SEM), X-ray photoelectron spectroscopy (XPS), contact angle measurement. were immobilized both groove bottom ridge top with a similar anchoring density. A fluorescence microscope visualized BMP-2 IGF-1 positive charges efficiently loaded negatively charged PAsp shells nanoparticles. confirmed enhanced MSCs presenting expression results major osteoblast marker genes proteins. In addition, overall significant correlations between experimental validity our study. proposed combined microgrooves factor-releasing can be used strong osteogenic promoter biomaterial surfaces.

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Surface functionalization of microgrooved titanium with dual growth factor-releasing nanoparticles for synergistic osteogenic differentiation of human mesenchymal stem cells

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Surface functionalization of microgrooved titanium with dual growth factor-releasing nanoparticles for synergistic osteogenic differentiation of human mesenchymal stem cells

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