Insulin-like growth factor-1 (IGF-1) enhances the osteogenic activity of bone morphogenetic protein-6 (BMP-6) in vitro and in vivo, and together have a stronger osteogenic effect than when IGF-1 is combined with BMP-2.

摘要: Bone morphogenetic protein-2 (BMP-2) is widely used in orthopedic surgery and bone tissue engineering because of its strong osteogenic activity. However, BMP-2 treatments have several drawbacks many groups are actively exploring alternatives. Since BMP-6 has been demonstrated to be more osteoinductive, use, either alone or together with other growth factors, might an interesting option. In this work, we compared the effect BMP-2, BMP-6, insulin-like factor-1 (IGF-1), combination. Murine preosteoblasts were treated 15 nM IGF-1 and/or 6 -6 expression marker genes, proliferation, alkaline phosphatase (ALP) activity vitro analyzed. The results showed that greatly enhanced BMP-induced differentiation these cells general ALP cultures was higher when combination made than BMP-2. Furthermore, tested potential vivo by loading 25 pmoles 10 onto absorbable collagen sponges implanting them into ectopic formation model rats. This study revealed only able induce at dose addition contributed increase mineralization implants. Hence, a better alternative for approaches. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1867-1875, 2017.