Hepatocyte growth factor is a potent angiogenic factor which stimulates endothelial cell motility and growth

摘要: Hepatocyte Growth Factor (HGF, also known as Scatter Factor) is a powerful mitogen or motility factor in different cells, acting through the tyrosine kinase receptor encoded by MET protooncogene. Endothelial cells express gene and expose at cell surface mature protein (p190MET) made of 50 kD (alpha) subunit disulfide linked to 145-kD (beta) subunit. HGF binding endothelial identifies two sites with affinities. The higher affinity site (Kd = 0.35 nM) corresponds p190MET receptor. Sub-nanomolar concentrations HGF, but not recombinant inactive precursor, stimulate activity, proliferation motility. induces repairs wound monolayer. stimulates scatter grown on three-dimensional collagen gels, inducing an elongated phenotype. In rabbit cornea, highly purified promotes neovascularization sub-nanomolar concentrations. lacks activities related hemostasis-thrombosis, inflammation accessory functions. These data show that vivo potent angiogenic vitro proliferate migrate.