Biosynthesis of the protein encoded by the c-met proto-oncogene.

摘要: Abstract The proto-oncogene c-met encodes a transmembrane protein with structural features of growth factor receptor. We have previously shown that the (c-Met) is heterodimer two disulphide linked chains 50 kd (alpha) and 145 (beta). In this work we studied biosynthesis product in gastric carcinoma cell line (GTL-16) where gene amplified overexpressed. Following metabolic labelling cells presence tunicamycin, anti-met antibodies immunoprecipitate 150 kd. pulse-chase experiments carried out absence 170 appears first. Within next few minutes, precursor modifies its SDS migration, probably as consequence modification(s) intra-chain bonds. After 45 min chase, single polypeptide cleaved to form alpha subunit beta are joined by bonds an complex apparent molecular weight 190 N-linked oligosaccharides both mature was enzymatic de-glycosylation immunoprecipitated proteins. half-life calculated be approximately 5h. has similar structure other human lines.