Flavonoids isolated from Citrus platymamma induce mitochondrial-dependent apoptosis in AGS cells by modulation of the PI3K/AKT and MAPK pathways.
作者： HO JEONG LEE , ARULKUMAR NAGAPPAN , HYEON SOO PARK , GYEONG EUN HONG , SILVIA YUMNAM
DOI: 10.3892/OR.2015.4122
关键词: PI3K/AKT/mTOR pathway 、 Protein kinase B 、 MAPK/ERK pathway 、 p38 mitogen-activated protein kinases 、 Biology 、 Cell biology 、 Apoptosis 、 Programmed cell death 、 Kinase 、 Cell growth
摘要: Citrus platymamma hort. ex Tanaka (Rutaceae family) has been widely used in Korean folk medicine for its wide range of medicinal benefits including an anticancer effect. In the present study, we aimed to investigate molecular mechanism effects flavonoids isolated from (FCP) on AGS cells. FCP treatment significantly inhibited cell growth a dose‑dependent manner. Furthermore, increased percentage cells sub-G1 phase (apoptotic population), and apoptosis was confirmed by Annexin V double staining. Chromatin condensation apoptotic bodies were also noted FCP-treated Moreover, immunoblotting results showed that decreased expression procaspase-3, -6, -8 -9, PARP cleaved caspase-3, Bax/Bcl-xL ratio dose-dependent addition, phosphorylation AKT decreased, whereas extracellular signal-related kinase 1/2 (ERK1/2), c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinases (MAPKs) Taken together, death response mitochondrial-dependent via modulation PI3K/AKT MAPK pathways. These findings provide new insight understanding FCP. Thus, may be potential chemotherapeutic agent gastric cancer.
spandidos.info参考文章(38)
Yigong Shi, Caspase Activation: Revisiting the Induced Proximity Model Cell. ,vol. 117, pp. 855- 858 ,(2004) , 10.1016/J.CELL.2004.06.007
Gary A. Koretzky, Shailaja Kasibhatla, Kevin Latinis, Mary Faris, Niels Kokot, Andre Nel, Douglas R. Green, The c-Jun N-terminal kinase cascade plays a role in stress-induced apoptosis in Jurkat cells by up-regulating Fas ligand expression. Journal of Immunology. ,vol. 160, pp. 134- 144 ,(1998)
Michael O. Hengartner, The biochemistry of apoptosis Nature. ,vol. 407, pp. 770- 776 ,(2000) , 10.1038/35037710
Murali Gururajan, Trivikram Dasu, Seif Shahidain, C. Darrell Jennings, Darrell A. Robertson, Vivek M. Rangnekar, Subbarao Bondada, Spleen tyrosine kinase (Syk), a novel target of curcumin, is required for B lymphoma growth. Journal of Immunology. ,vol. 178, pp. 111- 121 ,(2007) , 10.4049/JIMMUNOL.178.1.111
Jin-A. Kim, Hyeon-Soo Park, Sang-Rim Kang, Kwang-Il Park, Do-Hoon Lee, ArulKumar Nagappan, Sung-Chul Shin, Won-Sup Lee, Eun-Hee Kim, Gon-Sup Kim, Suppressive effect of flavonoids from Korean Citrus aurantium L. on the expression of inflammatory mediators in L6 skeletal muscle cells. Phytotherapy Research. ,vol. 26, pp. 1904- 1912 ,(2012) , 10.1002/PTR.4666
Julie Acunzo, Maria Katsogiannou, Palma Rocchi, Small heat shock proteins HSP27 (HspB1), αB-crystallin (HspB5) and HSP22 (HspB8) as regulators of cell death The International Journal of Biochemistry & Cell Biology. ,vol. 44, pp. 1622- 1631 ,(2012) , 10.1016/J.BIOCEL.2012.04.002
L Harrison, Extended lymphadenectomy is associated with a survival benefit for node-negative gastric cancer. Journal of Gastrointestinal Surgery. ,vol. 2, pp. 126- 131 ,(1998) , 10.1016/S1091-255X(98)80002-4
Dae-Hee Lee, Tae Hwa Lee, Chang Hwa Jung, Young-Ho Kim, Wogonin induces apoptosis by activating the AMPK and p53 signaling pathways in human glioblastoma cells. Cellular Signalling. ,vol. 24, pp. 2216- 2225 ,(2012) , 10.1016/J.CELLSIG.2012.07.019
Arulkumar Nagappan, Kwang Il Park, Hyeon Soo Park, Jin A Kim, Gyeong Eun Hong, Sang Rim Kang, Do Hoon Lee, Eun Hee Kim, Won Sup Lee, Chung Kil Won, Gon Sup Kim, Vitamin C induces apoptosis in AGS cells by down-regulation of 14-3-3σ via a mitochondrial dependent pathway. Food Chemistry. ,vol. 135, pp. 1920- 1928 ,(2012) , 10.1016/J.FOODCHEM.2012.06.050
Evropi Theodoratou, Janet Kyle, Roseanne Cetnarskyj, Susan M. Farrington, Albert Tenesa, Rebecca Barnetson, Mary Porteous, Malcolm Dunlop, Harry Campbell, Dietary flavonoids and the risk of colorectal cancer. Cancer Epidemiology, Biomarkers & Prevention. ,vol. 16, pp. 684- 693 ,(2007) , 10.1158/1055-9965.EPI-06-0785