Biocalorimetric and respirometric studies on production of Penicillin G acylase from Bacillus badius pac in E. coli DH5α

摘要: Abstract An isothermal heat flux biological reaction calorimeter is employed to monitor the production of Penicillin G acylase (PAC) from E. coli DH5α harboring recombinant construct pPROPAC (pET-30b carrying pac gene Bacillus badius with its own promoter) cultivated in fermentation medium earlier different flow rates oxygen. Three oxygen were studied. At 0.66 vvm (volume gas per volume liquid minute [l l−1 min−1]) a maximum enzyme yield 43 U/ml was achieved fructose as carbon source. Metabolic rate profile and yields at levels helped conclude that an obligate aerobic. Oxygen uptake correlated well activity, biomass growth exponential stationary phases optimized conditions. It observed metabolic heat, substrate utilization matched well. The due growth, 15.26 kJ/g, consumption, 16.52 kJ/g uptake, 413.2 kJ/mol led understanding process behavior physiological activity organism under study. results can contribute scale up studies, possibly.