Central bombesin possibly induces S-nitrosylation of cyclooxygenase-1 in pre-sympathetic neurons of rat hypothalamic paraventricular nucleus

摘要: Aims Cyclooxygenase (COX) can be activated by nitric oxide-induced (NO-induced) conversion of cysteine thiol group COX into S-nitrosothiol. We previously reported the involvement brain COX/NO synthase (NOS) in centrally administered bombesin-, a stress-related neuropeptide, induced secretion rat adrenal noradrenaline and adrenaline. To examine possible NO-induced modification bombesin-induced response, we investigated whether bombesin induces close proximity COX-1 neuronal NOS (nNOS) or S-nitroso-cysteine pre-sympathetic spinally projecting neurons hypothalamic paraventricular nucleus (PVN), regulatory center adrenomedullary outflow. Main methods In twelve-week-old male Wistar rats, PVN were labeled with retrograde tracer Fluoro-Gold (FG). After intracerebroventricular administration bombesin, performed double immunohistochemical analysis for Fos nNOS FG-labeled neurons. also fluorescent situ ligation assay (PLA) visualizing (< 40 nm) S-nitroso-cysteine. Key. findings Bombesin significantly increased number Fos-immunoreactive cells immunoreactivity. 7-Nitroindazole, selective inhibitor, abolished Fos-immunoreactivity COX-1-immunoreactive Bombesin PLA-positive signals indicating COX-1/nNOS COX-1/S-nitroso-cysteine neurons. Significance Centrally possibly S-nitrosylation through neurons, thereby activating during activation central outflow rat.