Study of Some Stages of Poliovirus Morphogenesis in MiO Cells

摘要: Five to seven minutes after addition of 14 C-amino acids poliovirus-infected MiO cells, radioactivity is found only in 5 S virus-specific structures. With increased time labeling, appears considerable amounts also the area. In presence guanidine blocking transition particles subsequent structures first detected zone and then accumulates predominantly particles. These results indicate that formation conversion a part their protein into reflect earliest stages morphogenesis poliovirus. contrast HeLa no 73 could be nor cells short incubation period (20 30 min) with radioactive amino acids, although 150 virions was observed. Addition infected leads accumulation particles; however, are not detected. After removal guanidine, increases Incubation isolated from sucrose gradient cytoplasmic extract resulted The obtained show modification late poliovirus cells.