In vitro assembly of polioviruses

摘要: Abstract Rough membranes obtained from poliovirus-infected HeLa cells have the capacity to assemble 14 S particles into 73 empty capsids in vitro . A corresponding fraction uninfected did not possess this activity. When labeled were incubated with smooth infected cells, a significant amount of radioactivity sedimented as heterogeneous material 30–70 region gradient. Sucrose gradient analysis C-labeled rough and membrane fractions lysed deoxycholate (DOC) demonstrated presence particles, capsids, 110 structure fraction. These structures found only trace amounts In absence DOC treatment, no any fractions. The on other hand, detected or treatment rough-membrane Therefore, it appears that are associated where they assembled and/or complete virions.