Expression of colony-stimulating factor genes by normal human mesothelial cells and human malignant mesothelioma cells lines in vitro.

摘要: We investigated normal human mesothelial cells and malignant mesothelioma cell lines for the ability to produce hematopoietic colony-stimulating factors (CSFs) in culture. Early passage cultures of diploid spontaneously expressed detectable levels M-CSF mRNA transcripts, but lacked transcripts GM-CSF or G-CSF. Exposure epidermal growth factor (EGF), lipopolysaccharide (LPS), tumor necrosis (TNF) induced expression G-CSF mRNA. The combination EGF TNF threefold more than did either alone. were only by EGF. Interleukin-1 beta (IL-1 beta) EGF, TNF, LPS inhibited hydrocortisone (HC). All tested also transcripts. However, contrast cells, two four autonomously without stimulation. Secretion biologically active CSFs was confirmed testing media conditioned various types examined. detection correlated well with presence CSF Northern analysis. These data indicate that (a) express culture; (b) is an essential cofactor optimal induction expression; (c) exposure inflammatory mediators such as increases IL-1 beta; (d) compared some exhibit aberrant gene multiple cytokines, including G-CSF, GM-CSF, beta, IL-6.