Potentiation of granulocyte colony-stimulating factor-induced mobilization of circulating progenitor cells by seven-day pretreatment with interleukin-3

摘要: Granulocyte colony-stimulating factor (G-CSF) as a single agent is increasingly used for the mobilization of peripheral blood progenitor cells (PBPCs) stem cell transplantation. In patients with perturbed hematopoiesis mobilizing capacity G-CSF alone may be inadequate. We have shown in rhesus monkeys that interleukin-3 (IL-3) pretreatment markedly potentiated increase PBPC numbers by subsequent administration granulocyte/macrophage-CSF (GM-CSF). Here we studied effect IL-3 on G-CSF-induced PBPCs 6 Hodgkin9s disease (n = 5) or non-Hodgkin9s lymphoma 1) who had low because previous chemotherapy. Patients were treated cycle 1 at dose 5 microgram/kg/d days and, after treatment-free interval, received 2 consisting 7 followed again days. increased mean number circulating colony-forming units-GM (CFU-GM) 21-fold, burst-forming units-erythroid (BFU- E) 9-fold, and CFU-mix 24-fold over values. Treatment did not mobilize itself but significantly all types leading to 56-, 15-, 46- fold baseline CFU-GM, BFU-E, numbers, respectively. whom leukapheresis was performed G- CSF target x 10(6)/kg CD34+ reached. However, IL-3/G-CSF combination obtained > =2 3 patients, including both inadequate collection alone. one patient adequate function mobilized progenitors could demonstration rapid trilineage engraftment infusion myeloablative Seven-day useful augment G-CSF. The seems allow procurement sufficient some cannot adequately