Nanoparticle colloidal stability in cell culture media and impact on cellular interactions

摘要: Nanomaterials are finding increasing use for biomedical applications such as imaging, diagnostics, and drug delivery. While it is well understood that nanoparticle (NP) physico-chemical properties can dictate biological responses interactions, has been difficult to outline a unifying framework directly link NP expected in vitro vivo outcomes. When introduced complex media containing electrolytes, proteins, lipids, etc., nanoparticles (NPs) subjected range of forces which determine their behavior this environment. One aspect systems often understated or overlooked aggregation. aggregation will significantly alter (dosimetry, uptake, cytotoxicity), fate (pharmacokinetics, toxicity, biodistribution). Thus, understanding the factors driving colloidal stability paramount. Furthermore, studying interactions with NPs at nanoscale level requires an interdisciplinary effort robust multiple characterization techniques. This review examines stability, various efforts stabilize media, methods characterize situ, provides discussion regarding cells.