Citrate-capped iron oxide nanoparticles impair the osteogenic differentiation potential of rat mesenchymal stem cells

摘要: Surface modification of iron oxide nanoparticles may cause unexpected impact upon interaction with cells, such as cytotoxicity and change in the differentiation potential stem cells. In this study, two kinds different surface chemistries, i.e. one its pristine form (P-NPs) without extra capping molecules other coated citrate (C-NPs), similar sizes, ∼10 nm, measured by transmission electron microscopy X-ray diffractometry, were prepared. Both P-NPs C-NPs aggregated to some extent water, hydrodynamic diameters 211.4 ± 29 128.6 6.3 zeta values +23.5 0.3 −49.6 0.5 mV, respectively. However, both NPs further a 260 5.5 214 6.4 nm slightly negative charge (∼−10 mV) cell media. After being incubated rat mesenchymal cells (MSCs) for 14 d, types showed uptake kinetics final intracellular content, 53.3 pg per 59.9 C-NPs, minimal at concentration below 100 μg mL−1. The adipogenic MSCs was unaltered regardless NP types, did not have an obvious on osteogenic MSCs. MSCs, however, significantly impaired incubation evidenced reduced expression markers, namely collagen type I (COL) osteocalcin (OCN) calcium deposition. surface-anchored found synergistic effect.