Codon optimization of the rabbit hemorrhagic disease virus (RHDV) capsid gene leads to increased gene expression in Spodoptera frugiperda 9 (Sf9) cells.

摘要: Rabbit hemorrhagic disease (RHD) is contagious and highly lethal. Commercial vaccines against RHD are produced from the livers of experimentally infected rabbits. Although several groups have reported that recombinant subunit rabbit virus (RHDV) promising, application has been restricted due to high production costs or low yield. In present study, we performed codon optimization capsid gene increase number preference codons eliminate rare in Spodoptera frugiperda 9 (Sf9) cells. The was then subcloned into pFastBac plasmid, baculoviruses were identified with a plaque assay. As expected, expression optimized protein markedly increased Sf9 cells, proteins self-assembled virus-like particles (VLPs) released cell supernatant. Rabbits inoculated supernatant purified VLPs protected RHDV challenge. A rapid, specific antibody response detected by an ELISA all experimental groups. conclusion, this strategy producing vaccine antigen can be used develop low-cost, insect cell-derived RHDV.