Mechanism of apoptosis induction in human breast cancer MCF-7 cell by Ruviprase, a small peptide from Daboia russelii russelii venom.
作者： Rupamoni Thakur , Sudarshan Kini , Sillarine Kurkalang , Atanu Banerjee , Purba Chatterjee
DOI: 10.1016/J.CBI.2016.09.004
关键词: Molecular biology 、 Cell 、 Caspase 7 、 HeLa 、 Peptide 、 Poly ADP ribose polymerase 、 Reactive oxygen species 、 Cancer cell 、 Apoptosis 、 Biology 、 Cell biology
摘要: Abstract Ruviprase, a 4.4 kDa peptide isolated from Daboia russelii venom demonstrated antiproliferative activity against EMT6/AR1, U-87MG, HeLa and MCF-7 cancer cells with an IC 50 value of 23.0, 8.8, 5.8 4.0 μg ml −1 , respectively. However, it was nontoxic to non-cancerous human embryonic kidney cell peripheral blood lymphocytes. Flow-cytometric analysis confirmed the apoptosis induction in by Ruviprase where induced DNA condensation but did not cause mitotic blockage or chromosomal aberration treated-cells. Immunofluorescence microscopic indicated through p53 p21-mediated pathways. generated reactive oxygen species (ROS), altered mitochondrial transmembrane potential, significantly decreased cellular glutathione (GSH) content cells. Immunoblotting quantitative real-time PCR (qRT-PCR) analyses suggested that down-regulated expression anti-apoptotic protein Bcl-2, increased cleavage poly (ADP-ribose) polymerase (PARP) protein, up-regulated pro-apoptotic Bax, as well executer caspase-7 via intrinsic pathway. This is first report on characterization anticancer potential small, non-toxic anticoagulant purified Russell's viper venom.
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