Semi-Automated Identification of N-Glycopeptides by Hydrophilic Interaction Chromatography, nano-Reverse-Phase LC–MS/MS, and Glycan Database Search
作者： Petr Pompach , Kevin B. Chandler , Renny Lan , Nathan Edwards , Radoslav Goldman
DOI: 10.1021/PR201183W
关键词: Mass spectrometry 、 Chemistry 、 Glycoprotein 、 Peptide 、 Hydrophilic interaction chromatography 、 Database search engine 、 Tandem mass spectrometry 、 Hemopexin 、 Chromatography 、 Glycan
摘要: Glycoproteins fulfill many indispensable biological functions, and changes in protein glycosylation have been observed various diseases. Improved analytical methods are needed to allow a complete characterization of this complex common post-translational modification. In study, we present workflow for the analysis microheterogeneity N-glycoproteins that couples hydrophilic interaction nanoreverse-phase C18 chromatography tandem QTOF mass spectrometric analysis. A glycan database search program, GlycoPeptideSearch, was developed match N-glycopeptide MS/MS spectra with glycopeptides comprised drawn from GlycomeDB structure peptide user-specified set potentially glycosylated peptides. Application human haptoglobin hemopexin, two microheterogeneous N-glycoproteins, identified total 57 distinct site-specific glycoforms case 14 hemopexin. Using g...
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sci-hub.se 参考文章(68)
Wanda Dobryszycka, Biological Functions of Haptoglobin - New Pieces to an Old Puzzle European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies. ,vol. 35, pp. 647- 654 ,(1997)
Katalin F. Medzihradszky, Characterization of protein N-glycosylation. Methods in Enzymology. ,vol. 405, pp. 116- 138 ,(2005) , 10.1016/S0076-6879(05)05006-8
Gabriel R. Drapeau, Yves Boily, Jean Houmard, Purification and Properties of an Extracellular Protease of Staphylococcus aureus Journal of Biological Chemistry. ,vol. 247, pp. 6720- 6726 ,(1972) , 10.1016/S0021-9258(19)44749-2
Jeffrey D Esko, Ajit Varki, Karen J Colley, Cellular Organization of Glycosylation Cold Spring Harbor Laboratory Press. ,(2009) , 10.1101/GLYCOBIOLOGY.3E.004
Caroline S. Chu, Milady R. Niñonuevo, Brian H. Clowers, Patrick D. Perkins, Hyun Joo An, Hongfeng Yin, Kevin Killeen, Suzanne Miyamoto, Rudolf Grimm, Carlito B. Lebrilla, Profile of native N-linked glycan structures from human serum using high performance liquid chromatography on a microfluidic chip and time-of-flight mass spectrometry. Proteomics. ,vol. 9, pp. 1939- 1951 ,(2009) , 10.1002/PMIC.200800249
Habtom W. Ressom, Rency S. Varghese, Lenka Goldman, Yanming An, Christopher A. Loffredo, Mohamed Abdel-Hamid, Zuzana Kyselova, Yehia Mechref, Milos Novotny, Steven K Drake, Radoslav Goldman, Analysis of MALDI-TOF mass spectrometry data for discovery of peptide and glycan biomarkers of hepatocellular carcinoma. Journal of Proteome Research. ,vol. 7, pp. 603- 610 ,(2008) , 10.1021/PR0705237
Radoslav Goldman, Habtom W. Ressom, Rency S. Varghese, Lenka Goldman, Gregory Bascug, Christopher A. Loffredo, Mohamed Abdel-Hamid, Iman Gouda, Sameera Ezzat, Zuzana Kyselova, Yehia Mechref, Milos V. Novotny, Detection of hepatocellular carcinoma using glycomic analysis. Clinical Cancer Research. ,vol. 15, pp. 1808- 1813 ,(2009) , 10.1158/1078-0432.CCR-07-5261
Federico Di Rocco, Yuichiro Nonaka, Hideo Hamada, Masami Yoshino, Hiromichi Nakazaki, Shizuo Oi, Endoscopic biopsy interpretation difficulties in a congenital diffuse intracranial teratoma. Childs Nervous System. ,vol. 22, pp. 84- 89 ,(2006) , 10.1007/S00381-004-1088-Y
David Goldberg, Mark Sutton-Smith, James Paulson, Anne Dell, Automatic annotation of matrix-assisted laser desorption/ionization N-glycan spectra. Proteomics. ,vol. 5, pp. 865- 875 ,(2005) , 10.1002/PMIC.200401071
Michael J. Huddleston, Mark F. Bean, Steven A. Carr, Collisional fragmentation of glycopeptides by electrospray ionization LC/MS and LC/MS/MS: methods for selective detection of glycopeptides in protein digests. Analytical Chemistry. ,vol. 65, pp. 877- 884 ,(1993) , 10.1021/AC00055A009