Gangliosides as receptors for fibronectin? Comparison of cell spreading on a ganglioside-specific ligand with that on fibronectin.

摘要: Abstract We have examined the possibility that gangliosides act as cell surface receptor for fibronectin, previously suggested by data of Kleinman et al. (Proc natl acad sci US 76 (1979) 3367), using three different approaches. 1. Gangliosides inhibited spreading both CHO and BHK cells on fibronectin-coated substrates. 50% inhibition was produced 1.0 3.5 × 10 −5 M di- trisialogangliosides respectively, although monosialogangliosides were less effective inhibitors. The apparently due to an interaction with fibronectin not a direct effect cells. 2. Using anti-fibronectin antibodies, 125 I-labelled protein A, adsorbed polystyrene tubes, we provided evidence will bind gangliosides. However, is low affinity compared binding cholera toxin 3. ability BALB/c 3T3 spread substrates coated ganglioside-specific ligand, B-subunit. Cells plated onto rapidly (within 60 min) adopted well morphology, whereas B-subunit extensive even after 2 h. In addition, organization F-actin within two types substrate also quite different. conclude may be influenced membrane-bound It unlikely, however, such can lead cytoskeletal reorganization which characteristic fibronectin.