PGE2 INDUCES THE TRANSITION FROM NON-ADHERENT TO ADHERENT BONE MARROW MESENCHYMAL PRECURSOR CELLS VIA A CAMP/EP2-MEDIATED MECHANISM
作者： A. Scutt , M. Zeschnigk , P. Bertram
DOI: 10.1016/0090-6980(95)00070-Q
关键词: Osteoblast 、 Biology 、 Bucladesine 、 Forskolin 、 Bone marrow 、 Endocrinology 、 Inositol phosphate 、 Internal medicine 、 Cell biology 、 Protein kinase A 、 Phorbol 、 IBMX
摘要: When mesenchymal precursor cells from bone marrow are cultured in the presence of dexamethasone, existence distinct non-adherent and adherent populations can be demonstrated. The addition PGE2, forskolin, or dibutyryl-cAMP induce a transition former to latter this may an important mechanism anabolic effects PGE2. On other hand, phorbol 12-myristate 13-acetate (PMA), activator protein kinase C, sulprostone, agonist for PGE2 receptor EP1/EP3 subtypes, had no effect. phosphodiesterase inhibitor, isobutylmethylxanthine (IBMX), synergistic effect combination with whereas neomycin, inhibitor inositol phosphate activity, effect, LiC1, triphosphate metabolism, inhibitory on PGE2-induced transition. Consistent this, caused 100% increase cAMP synthesis. These results suggest that induction osteoblast is mediated by EP2-PGE2 subtype via intracellular
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