Monoclonal antibodies against surface determinants on gametes of Plasmodium gallinaceum block transmission of malaria parasites to mosquitoes.

摘要: Monoclonal antibodies (MAb) against gametes of the chicken malaria Plasmodium gallinaceum have been derived. All reacted with surface extracellular parasite in immunofluorescent antibody reactions and all agglutinated both male female gametes. In absence active complement one mu isotype MAb, la 1-D5, mediated at least 95% suppression infectivity parasites to Aedes aegypti mosquitoes. Individually, MAb gamma 1 or 2a isotypes only slight complement. Certain combinations these however, suppressed by 90 95%. Suppression was shown be mainly due their effects on events leading up including fertilization. which otherwise minimal no suppressive effect, completely abolished if present. No target antigen could identified immunoprecipitation Triton X-100 extracts radioiodinated zygotes P. for MAb. precipitated same three proteins 240,000, 56,000, 54,000 daltons under reducing conditions sodium dodecyl sulfate-polyacrylamide gel electrophoresis, from These sexes thus appear include antigens anti-gamete transmission blocking immunity.