The conglomeration of diagnostic, prognostic and therapeutic potential of serum miR-199a and its association with clinicopathological features in epithelial ovarian cancer

摘要: Epithelial ovarian cancer (EOC) is the most lethal cause of morbidity and mortality worldwide. miRNA deregulation evinces a remarkable role in tumorigenesis. miRNA-199a (miR-199a) known to be involved development progression. Although miR-199a has been studied various cell types, its correlation with clinicopathological features EOC not documented. In this study, we identified hallmarks which might perturbed due downregulation serum EOC. Seventy samples from histopathologically confirmed patients 70 controls were collected. Total RNA was isolated by Trizol method, polyadenylated reverse transcribed into cDNA. Expression level detected using qRT-PCR. Relative expression determined matched U6 snRNA as reference. Level compared distinct features. found significantly downregulated comparison normal controls. The associated tumor stage, lymph node metastasis, distal metastasis. Receiver operating characteristic (ROC) curve for diagnostic potential yielded significant area under (AUC) considerable sensitivity specificity. ROC curves prognosis AUCs histological grade, status, survival. Our findings suggest that indicator disease progression promoting aggressive marker predict survival patients.