Serum miR-21, miR-29a and miR-125b are promising biomarkers for the early detection of colorectal neoplasia

摘要: Purpose: Circulating microRNAs (miRNA) are emerging as promising diagnostic biomarkers for colorectal cancer, but their usefulness detecting early neoplasms remains unclear. This study aimed to identify serum miRNA the identification of patients with neoplasms. Experimental Design: A cohort 237 samples from 160 (148 precancerous lesions and 12 cancers) 77 healthy subjects was analyzed in a three-step approach that included comprehensive literature review published biomarkers, screening phase, validation phase. RNA extracted sera, levels miRNAs were examined by real-time RT-PCR. Results: Nine (miR-18a, miR-19a, miR-19b, miR-20a, miR-21, miR-24, miR-29a, miR-92, miR-125b) selected candidate initial analysis. In miR-125b significantly higher neoplasm than controls. Elevated confirmed phase using an independent set subjects. Area under curve (AUC) values miR-125b, combined score discriminating controls 0.706, 0.741, 0.806, 0.827, respectively. Serum miR-29a who had only small (≤5 mm) Conclusions: Because discriminated controls, our data highlight potential clinical use these molecular signatures noninvasive neoplasia. Clin Cancer Res; 21(18); 4234–42. ©2015 AACR .