PDCD4 nuclear loss inversely correlates with miR-21 levels in colon carcinogenesis

摘要: Programmed cell death 4 (PDCD4) has recently been demonstrated to be a new tumor suppressor gene involved in colon carcinogenesis. PDCD4 immunohistochemical expression was assessed 300 polypoid lesions of the mucosa (50 hyperplastic polyps [HP], 50 serrated adenomas [SA], tubular with low-grade-intraepithelial neoplasia [LG-IEN], high-grade-IEN [HG-IEN]), and adenocarcinomas (CRC). As normal controls, we considered biopsy samples obtained from patients irritable bowel syndrome (N). We further investigated messenger RNA (mRNA) levels by quantitative real-time polymerase chain reaction (PCR) different series N, LG-IEN, HG-IEN, CRC samples. miR-21 (an important PDCD4-expression regulator) also determined PCR situ hybridization. Normal colocytes HP featured strong nuclear immunostaining whereas significantly lower observed dysplasia (low- high-grade SA) invasive CRC. mRNA decreased as phenotypic changes occurring during carcinogenesis progressively increased (p < 0.001). expected, upregulated preneoplastic/neoplastic samples, consistent downregulation. These results consistently support use downregulation novel biomarker for diagnosis dysplastic/neoplastic